Born in Brussels to Greek parents, Daphné Patakia grew up in Belgium before going to Athens to study comedy. After leaving the Greek National Theater in 2013, she took her first steps as an actress in several local films. She was notably awarded a Shooting Stars award at the 2016 Berlinale for her role as a delinquent in The Awakening of Spring by Constantínos Giánnaris.

The following year, Daphné Patakia moves to Paris to develop her international career. After a few secondary characters, she gets her first role in Djam, a Franco-Greek-Turkish drama directed by Tony Gatlif. She plays the heroine, sent to Istanbul by her uncle in search of a missing part of a boat, in a remarkable performance that earned her the shortlist for the César for Most Promising Actress in 2018.

In 2020, Daphné Patakia appears for the first time on the small screen in the adaptation of Tanguy Viel's novel, Paris-Brest, in which she plays the protagonist's girlfriend. At the same time, the actress is on the bill for Benedetta, the new feature film by Paul Verhoeven, selected at the 2021 Cannes Film Festival. It is an opportunity for her to work alongside renowned French actors such as Virginie Efira and Lambert Wilson.

In 2021, the young woman is also announced in the cast of the comedy-drama The Five Devils, directed by George Brianka, Edwin M. Figueroa e Terry R. Wickham, and the thriller Everyone Calls Me Mike, alongside Pierre Lottin.